Description
This course will examine molecular and mechanistic aspects of cell biology. Topics include: cell biochemistry and biosynthesis, cell signaling, regulation of the cell cycle and membrane trafficking. This course will also cover applied concepts and research techniques in molecular biology. It is designed for students with a good basic knowledge of molecular biology who want to study more advanced concepts and how they may be applied in biotechnology. Topics for discussion include; Introduction to Microbiology, DNA and Molecular Biology, Basic Cloning Requirements, Gene Cloning Techniques, Molecular Hybridisation (Northern, Southern and Western), Construction and screening of Genomic and cDNA Libraries, Restriction Fragment Mapping, Design and use of Oligonucleotide Primers, DNA Sequencing, Polymerase Chain Reaction and RT-PCR, Site-directed Mutagenesis, Cloning Vectors and their Applications, Gene Expression Systems, Bioinformatics and Database Searching, Microarrays Technology.